The caudo-ventral pallium is a novel pallial domain expressing Gdf10 and generating Ebf3-positive neurons of the medial amygdala

Brain Struct Funct. 2018 Sep;223(7):3279-3295. doi: 10.1007/s00429-018-1687-0. Epub 2018 Jun 4.


In rodents, the medial nucleus of the amygdala receives direct inputs from the accessory olfactory bulbs and is mainly implicated in pheromone-mediated reproductive and defensive behaviors. The principal neurons of the medial amygdala are GABAergic neurons generated principally in the caudo-ventral medial ganglionic eminence and preoptic area. Beside GABAergic neurons, the medial amygdala also contains glutamatergic Otp-expressing neurons cells generated in the lateral hypothalamic neuroepithelium and a non-well characterized Pax6-positive population. In the present work, we describe a novel glutamatergic Ebf3-expressing neuronal subpopulation distributed within the periphery of the postero-ventral medial amygdala. These neurons are generated in a pallial domain characterized by high expression of Gdf10. This territory is topologically the most caudal tier of the ventral pallium and accordingly, we named it Caudo-Ventral Pallium (CVP). In the absence of Pax6, the CVP is disrupted and Ebf3-expressing neurons fail to be generated. Overall, this work proposes a novel model of the neuronal composition of the medial amygdala and unravels for the first time a new novel pallial subpopulation originating from the CVP and expressing the transcription factor Ebf3.

Keywords: Caudo-ventral pallium; Ebf3; Gdf10; Medial amygdala; Pax6; Telencephalon; Ventral pallium.

MeSH terms

  • Animals
  • Basal Forebrain / embryology
  • Basal Forebrain / metabolism*
  • Cell Lineage
  • Corticomedial Nuclear Complex / embryology
  • Corticomedial Nuclear Complex / metabolism*
  • Gene Expression Regulation, Developmental
  • Gestational Age
  • Glutamic Acid / metabolism
  • Growth Differentiation Factor 10 / genetics
  • Growth Differentiation Factor 10 / metabolism*
  • Mice, Inbred C57BL
  • Mice, Inbred ICR
  • Mice, Knockout
  • Neural Stem Cells / metabolism*
  • Neurons / metabolism*
  • PAX6 Transcription Factor / genetics
  • PAX6 Transcription Factor / metabolism
  • Tissue Culture Techniques
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*


  • Ebf3 protein, mouse
  • Gdf10 protein, mouse
  • Growth Differentiation Factor 10
  • PAX6 Transcription Factor
  • Pax6 protein, mouse
  • Transcription Factors
  • Glutamic Acid