Corneal chemical burn treatment through a delivery system consisting of TGF-β1 siRNA: in vitro and in vivo

Abstract

Chemical burns are major causes of corneal blindness. Transforming growth factor beta-1 (TGFβ₁) plays an important role in induction of corneal inflammation-related-fibrosis leading to the blindness. Here, a topical delivery system consisting anti-fibrotic TGF-β₁ siRNA, an inflammatory suppressing gene, was designed for treatment of corneal injuries. TGF-β₁ siRNA loaded in nanoparticles (NPs) made up of polyethyleneimine polymer demonstrated high fibroblast transfection efficiency. Moreover, TGF-β₁ and PDGF genes and ECM deposition were suppressed in isolated human corneal fibroblasts. NPs inhibited proliferation and transformation of fibroblasts to myofibroblasts by S-phase arrest and α-SMA suppression in vitro, respectively. The mentioned finding was also confirmed in vivo, addressing high wound-healing potential of prepared gene delivery system which was superior to conventional betamethasone treatment. Besides, CD4⁺ and α-SMA antibody staining showed inhibited angiogenesis and myofibroblast accumulation in treated corneas. This study opens a new way for treating corneal fibrosis through topical siRNA delivery.

Keywords: Angiogenesis; Corneal haze; Myofibroblasts; Polyethyleneimine; TGF-β1 siRNA.