To determine whether the clitoral infrafrenulum layer exists. This descriptive observational study was conducted using 15 fresh human female cadavers. Macro and micro stratum-by-stratum anatomical dissections of the clitoral frenulum were performed bilaterally. Target tissue biopsies were obtained for microscopic examination and the specimens were stained with eosin and hematoxylin. The skin folds with erectile tissue between them create the superficial stratum of the clitoral frenulum. The inferior edge of the skin fold is firmly fused with the superficial infrafrenulum fascial bundle, which creates the deep stratum. Histological examination confirmed that the clitoral frenulum comprises two layers: 1) the superficial stratum consists of the dermis, sub-dermis with cutis appendages (sebaceous and apocrine glands) and the squamous mucosa with erectile tissue between the skin folds; 2) the infrafrenulum fascia bundle consists of fibro-connective-adipose tissue with intermittent layers of adipose cells and fibrous bands. The clitoral infrafrenulum fascial bundle exists and creates the deep stratum of the clitoral frenulum. Histologically, the clitoral frenulum consists of two layers, the superficial and deep strata. Both strata form the clitoral frenulum stabilizing mechanism. This anatomical discovery has potential for immediate clinical implementation. Clin. Anat. 31:907-912, 2018. © 2018 Wiley Periodicals, Inc.
Keywords: clitoral frenulum; clitoral frenulum anatomy; clitoris; female; female genitalia; female organs; labium minus; vulva.
© 2018 Wiley Periodicals, Inc.