Proteomics is the large-scale analysis of proteins rendered possible by modern mass spectrometry analysis methods capable of identifying thousands of peptides/proteins in a fast high-throughput manner. Here I describe protocols for the preparation of fungal culture protein samples for mass spectrometry-based proteomics analysis including protein sample cleanup, proteolytic digestion, LC-MS/MS separation, and database search protein identification.
Keywords: Database search; Fungal proteome; In-gel digestion; In-solution digestion; LC-MS/MS; Microcapillary column packing.