Characterization of a mouse interferon gene locus I. Isolation of a cluster of four alpha interferon genes

Nucleic Acids Res. 1985 Feb 11;13(3):805-23. doi: 10.1093/nar/13.3.805.

Abstract

A BALB/c mouse genomic library was screened with a murine interferon alpha 2 (MuIFN-alpha 2) cDNA coding region fragment. Eight clones were isolated which contain different mouse chromosomal segments related to the MuIFN-alpha 2 probe and a 28 kilobase (kb) region of mouse genomic DNA containing four different MuIFN-alpha genes (alpha 1, alpha 4, alpha 5 and alpha 6) was identified and characterized; an intergenic 1000 nucleotide long conserved sequence was found to be associated with three of these four alpha genes, indicating that this alpha-IFN gene cluster evolved through tandem duplications. Sequence analysis revealed the absence of a polyadenylation site in the 3' untranslated region of MuIFN-alpha 1, and showed that one of the genes (alpha 4) contains an internal deletion of 5 amino acids in the coding region.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Mapping*
  • DNA Restriction Enzymes / metabolism
  • Interferon Type I / genetics*
  • Mice
  • Mice, Inbred BALB C
  • Repetitive Sequences, Nucleic Acid

Substances

  • Interferon Type I
  • DNA Restriction Enzymes

Associated data

  • GENBANK/M23828
  • GENBANK/M23829
  • GENBANK/M23830
  • GENBANK/M23831
  • GENBANK/M23925
  • GENBANK/M23926