Systematic metabolic profiling and bioactivity assays for bioconversion of Aceraceae family

PLoS One. 2018 Jun 7;13(6):e0198739. doi: 10.1371/journal.pone.0198739. eCollection 2018.

Abstract

Plants are an important and inexhaustible source of bioactive molecules in food, medicine, agriculture, and industry. In this study, we performed systematic liquid chromatography-mass spectrometry (LC-MS)-based metabolic profiling coupled with antioxidant assays for indigenous plant family extracts. Partial least-squares discriminant analysis of LC-MS datasets for the extracts of 34 plant species belonging to the families Aceraceae, Asteraceae, and Rosaceae showed that these species were clustered according to their respective phylogenies. In particular, seven Aceraceae species were clearly demarcated with higher average antioxidant activities, rationalizing their application for bioconversion studies. On the basis of further evaluation of the interspecies variability of metabolic profiles and antioxidant activities among Aceraceae family plants, we found that Acer tataricum (TA) extracts were clearly distinguished from those of other species, with a higher relative abundance of tannin derivatives. Further, we detected a strong positive correlation between most tannin derivatives and the observed higher antioxidant activities. Following Aspergillus oryzae-mediated fermentative bioconversion of Acer plant extracts, we observed a time-correlated (0-8 days) linear increase in antioxidant phenotypes for all species, with TA having the highest activity. Temporal analysis of the MS data revealed tannin bioconversion mechanisms with a relatively higher abundance of gallic acid (m/z 169) accumulated at the end of 8 days, particularly in TA. Similarly, quercetin precursor (glycoside) metabolites were also transformed to quercetin aglycones (m/z 301) in most Acer plant extracts. The present study underscores the efficacy of fermentative bioconversion strategies aimed at enhancing the quality and availability of bioactive metabolites from plant extracts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acer / chemistry*
  • Acer / metabolism*
  • Antioxidants / chemistry
  • Antioxidants / metabolism
  • Aspergillus oryzae / growth & development*
  • Chromatography, Liquid
  • Gallic Acid / chemistry
  • Gallic Acid / metabolism
  • Mass Spectrometry
  • Metabolome*
  • Metabolomics
  • Plant Extracts / chemistry*
  • Quercetin / chemistry
  • Quercetin / metabolism
  • Tannins / chemistry
  • Tannins / metabolism

Substances

  • Antioxidants
  • Plant Extracts
  • Tannins
  • Gallic Acid
  • Quercetin

Grant support

This paper was supported by Konkuk University in 2016.