Plasmids Related to the Broad Host Range Vector, pRK290, Useful for Gene Cloning and for Monitoring Gene Expression

Plasmid. 1985 Mar;13(2):149-53. doi: 10.1016/0147-619x(85)90068-x.


Derivatives of plasmid pRK290 that are useful for cloning and for analyzing gene expression in a wide variety of Gram-negative bacteria are described. A smaller broad host range plasmid derived from RK2, with properties similar to that of pRK290, is also described.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Gene Expression Regulation*
  • Genes, Bacterial*
  • Genetic Vectors*
  • Lac Operon
  • Plasmids*
  • beta-Galactosidase / genetics


  • DNA Restriction Enzymes
  • beta-Galactosidase