Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation

BMC Res Notes. 2018 Jun 7;11(1):361. doi: 10.1186/s13104-018-3455-x.


Objective: This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay.

Results: We have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma species commonly contaminating chlamydial stocks do not survive passage in mice. Chlamydia may also be derived Mycoplasma-free by plaque assay.

Keywords: Cell culture; Chlamydia; Contamination; Mycoplasma; Plaque assay.

MeSH terms

  • Animals
  • Chlamydia*
  • Female
  • Genetic Techniques*
  • HeLa Cells
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Microbiological Techniques / methods*
  • Mycoplasma*
  • Polymerase Chain Reaction