Components in Lentinus edodes mushroom with anti-biofilm activity directed against bacteria involved in caries and gingivitis

Food Funct. 2018 Jun 20;9(6):3489-3499. doi: 10.1039/c7fo01727h.


The present study investigated the compounds present in the low molecular mass fraction of Lentinus edodes mushroom (shiitake) extract and their anti-virulence activity against oral pathogens (reference and clinical Streptococcus mutans, Actinomyces naeslundii, and Prevotella intermedia strains). Oxalic, succinic, and quinic acids, and adenine, inosine, and uridine were identified by HPLC-DAD-ESI-MS/MS. Their anti-biofilm production and preformed biofilm disaggregation activities were studied using commercial standard compounds at different concentrations. As regards S. mutans, the highest activity was shown by adenine at 5 mg mL-1 both in the biofilm inhibition (BI 50%) and biofilm disaggregation tests (BD 20%). Considering A. naeslundii, BI values close to 80% were registered for oxalic acid at 1 mg mL-1 and 2 mg mL-1 and BD 50% for quinic acid at 3 mg mL-1. A weaker activity was found against P. intermedia. Furthermore, different mixtures of the commercial standards were tested showing that the activity of a compound can be strongly and sometimes negatively affected by the presence of the other compounds.

MeSH terms

  • Actinomyces / drug effects*
  • Actinomyces / physiology
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / pharmacology*
  • Biofilms / drug effects*
  • Chromatography, High Pressure Liquid
  • Dental Caries / microbiology*
  • Gingivitis / microbiology*
  • Humans
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Prevotella intermedia / drug effects*
  • Prevotella intermedia / physiology
  • Shiitake Mushrooms / chemistry*
  • Streptococcus mutans / drug effects*
  • Streptococcus mutans / physiology
  • Tandem Mass Spectrometry


  • Anti-Bacterial Agents
  • Plant Extracts