A facile brain drug delivery system for Alzheimer's disease, the Magnetic iron oxide (Fe3O4) nanoparticles loaded with polyethylene glycol-polylactide-polyglycolide co-polymer nanocomposite was fabricated with anti-transferrin monoclonal antibody (OX26) receptor in this present research work with its in vitro delivery properties evaluated by using clioquinol. TEM microscopic analysis confirmed the presence of active MNPs on the surface of OX26-PEG/PLGA nanocomposite matrix. The results of in vitro cell cytocompatibility studies of MNPs incorporated OX26-PEG/PLGA matrix showed a more favorable environment and increasing cell viability in normal human neuroblastoma SH-SY5Y cells than that of raw MNPs and PEG/PLGA co-polymer matrix. All these structural and in vitro studies results revealed that MNPs-PEG/PLGA-OX26 nanocomposite was a promising brain drug delivery system and it's potential for the Alzheimer's diseases.