Expression and prognostic value of cell-cycle-associated genes in gastric adenocarcinoma

BMC Gastroenterol. 2018 Jun 8;18(1):81. doi: 10.1186/s12876-018-0811-1.


Background: Gastric carcinoma is a malignant disease, and gastric adenocarcinoma (GAC) is the most common histological type. Molecular profiling of GAC has been extensively performed, but few have focused on the clinical significance of gene clusters of the cell cycle.

Methods: We investigated the genetic profile of cell-cycle-associated genes in a GAC cohort. The mRNA expression and clinical data were downloaded from TCGA, according to cBioportal. We conducted a series of analyses to detect the relationships between these genes and GAC.

Results: From all the patients, 5 clusters were identified based on mRNA expression of 122 cell-cycle-associated genes. Cluster 1 showed the worst prognosis and is characterized by extremely high expression of WEE2 and CCNE1. Comparison of the gene patterns showed that 16 genes expressed were distinctly varied between each cluster. In addition, investigations into the prognostic role of the 16 genes suggested that high expression of ESPL1 and MCM5 were significantly correlated with favorable outcomes. Moreover, we detected that ESPL1 and MCM5 gene expression were negatively correlated with GAC pathologic stage progression.

Conclusions: This study revealed a gene expression pattern of the cell cycle in different GAC subgroups, and suggested individual genes were associated with the clinical outcome and AJCC stages. These results suggest a novel prognostic strategy for GAC and provide information for patient stratification and trials of targeted therapies.

Keywords: Cell cycle; Gastric adenocarcinoma; Gene expression; Prognostic implications; Tumor stages.

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Aged
  • Cell Cycle Proteins / genetics
  • Cyclin E / genetics
  • Disease Progression
  • Female
  • Gene Expression
  • Gene Expression Profiling
  • Genes, cdc*
  • Humans
  • Male
  • Middle Aged
  • Multigene Family
  • Neoplasm Staging
  • Oncogene Proteins / genetics
  • Prognosis
  • Protein-Tyrosine Kinases / genetics
  • RNA, Messenger / genetics
  • Separase / genetics
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology
  • Survival Analysis


  • CCNE1 protein, human
  • Cell Cycle Proteins
  • Cyclin E
  • MCM5 protein, human
  • Oncogene Proteins
  • RNA, Messenger
  • Wee2 protein, human
  • Protein-Tyrosine Kinases
  • ESPL1 protein, human
  • Separase