CRISPR-FRT Targets Shared Sites in a Knock-Out Collection for Off-The-Shelf Genome Editing

Nat Commun. 2018 Jun 8;9(1):2231. doi: 10.1038/s41467-018-04651-5.

Abstract

CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad platform for fast, scarless, off-the-shelf genome engineering.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Binding Sites / genetics
  • CRISPR-Cas Systems*
  • DNA Nucleotidyltransferases / genetics
  • DNA Nucleotidyltransferases / metabolism*
  • DNA, Bacterial / genetics
  • DNA, Bacterial / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Editing / methods*
  • Gene Knockout Techniques
  • Genome, Bacterial / genetics
  • Models, Genetic
  • Mutation
  • RNA, Guide / genetics
  • RNA, Guide / metabolism*

Substances

  • DNA, Bacterial
  • RNA, Guide
  • DNA Nucleotidyltransferases
  • Site-specific recombinase