Kinetic Analysis of Amyloid Formation

Methods Mol Biol. 2018:1779:181-196. doi: 10.1007/978-1-4939-7816-8_12.

Abstract

The formation of amyloid fibrils is a central phenomenon in the progressive pathology of many neurodegenerative diseases, as well as in the fabrication of functional materials. Several different molecular processes acting in concert are responsible for the formation of amyloid fibrils from monomeric protein in solution. Here, we describe a method to determine which microscopic processes drive the overall formation of fibrils by using chemical kinetics in combination with systematic experimental datasets analysed in a global manner. We outline general concepts for obtaining suitable kinetic data and detail the key stages of data analysis, from quality control to the verification of a specific mechanism of aggregation.

Keywords: Amyloid fibrils; Kinetic modeling; Molecular-level mechanism; Protein aggregation; Self-assembly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid / chemistry*
  • Amyloid / metabolism*
  • Humans
  • Kinetics
  • Models, Molecular
  • Neurodegenerative Diseases / metabolism*
  • Protein Aggregates
  • Protein Multimerization

Substances

  • Amyloid
  • Protein Aggregates