Luminescent-Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues

K Peter R Nilsson; Mikael Lindgren; Per Hammarström

doi:10.1007/978-1-4939-7816-8_30

Luminescent-Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues

Methods Mol Biol. 2018:1779:485-496. doi: 10.1007/978-1-4939-7816-8_30.

Authors

K Peter R Nilsson¹, Mikael Lindgren^{1

2}, Per Hammarström³

Affiliations

¹ IFM-Department of Chemistry, Linköping University, Linköping, Sweden.
² Department of Physics, The Norwegian University of Science and Technology, 7491, Trondheim, Norway.
³ IFM-Department of Chemistry, Linköping University, Linköping, Sweden. per.hammarstrom@liu.se.

PMID: 29886552
DOI: 10.1007/978-1-4939-7816-8_30

Abstract

Luminescent-conjugated oligo- and polythiophenes (LCOs and LCPs) are valuable tools for optical imaging of a plethora of protein aggregates associated with amyloidoses. Here, we outline updated protocols for the application of the anionic pentameric LCO, p-FTAA, for staining and hyperspectral imaging of protein aggregates in a variety of settings such as in vitro formed amyloid fibrils, ex vivo tissue sections, and whole brain Drosophila.

Keywords: Amyloid; Fluorescence imaging; Luminescent-conjugated oligothiophenes; Protein aggregates; p-FTAA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amyloid / chemistry*
Amyloid / metabolism*
Animals
Brain / diagnostic imaging*
Brain / metabolism
Drosophila Proteins / metabolism
Drosophila melanogaster
Models, Molecular
Optical Imaging
Protein Aggregates
Protein Multimerization
Staining and Labeling
Thiophenes / metabolism*

Substances

Amyloid
Drosophila Proteins
Protein Aggregates
Thiophenes