no blokes Is Essential for Male Viability and X Chromosome Gene Expression in the Australian Sheep Blowfly

Abstract

It has been hypothesized that the Drosophila 4^th chromosome is derived from an ancient X chromosome [1]. In the Australian sheep blowfly, Lucilia cuprina, the heterochromatic X chromosome contains few active genes and orthologs of Drosophila X-linked genes are autosomal. Of 8 X-linked genes identified previously in L. cuprina, 6 were orthologs of Drosophila 4^th-chromosome genes [2]. The X-linked genes were expressed equally in males and females. Here we identify an additional 51 X-linked genes and show that most are dosage compensated. Orthologs of 49 of the 59 X-linked genes are on the 4^th chromosome in D. melanogaster. Because painting of fourth (Pof) is important for expression of Drosophila 4^th-chromosome genes [3], we used Cas9 to make a loss-of-function knockin mutation in an L. cuprina Pof ortholog we call no blokes (nbl). Homozygous nbl males derived from homozygous nbl mothers die at the late pupal stage. Homozygous nbl females are viable, fertile, and live longer than heterozygous nbl females. RNA expression of most X-linked genes was reduced in homozygous nbl male pupae and to a lesser extent in nbl females compared to heterozygous siblings. The results suggest that NBL could be important for X chromosome dosage compensation in L. cuprina. NBL may also facilitate gene expression in the heterochromatic environment of the X chromosome in both sexes. This study supports the hypothesis on the origin of the Drosophila 4^th chromosome and that a POF-like protein was required for normal gene expression on the ancient X chromosome.

Keywords: CRISPR/Cas9; Lucilia cuprina; dosage compensation; gene drive; male-specific lethal; painting of fourth; sex chromosome evolution; sheep blowfly.