Cyclic-AMP-dependent protein kinase type II is associated with the Golgi complex and with centrosomes

Cell. 1985 Jul;41(3):1039-51. doi: 10.1016/s0092-8674(85)80084-2.

Abstract

The subcellular distribution of the type II enzyme of cAMP-dependent protein kinase (cAMP-dPK II) in epithelial and fibroblastic cells was determined by indirect immunofluorescence microscopy. In interphase cells both regulatory (R II) and catalytic (C) subunits were concentrated in a perinuclear area. By comparison of the R II distribution with the location of a bona fide Golgi membrane constituent, this area was identified as the Golgi complex. The cytochemical localization of R II was confirmed by subcellular fractionation. In addition, cAMP-dPK II was associated with microtubule-organizing centers, in particular with mitotic spindle poles. These distributions of cAMP-dPK II probably represent important factors in mediating the effects of cAMP on basic cellular activities ranging from secretion and proliferation to cell shape and motility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology
  • Cattle
  • Cell Fractionation
  • Cell Line
  • Cyclic AMP / pharmacology
  • Epithelium
  • Fibroblasts
  • Fluorescent Antibody Technique
  • Golgi Apparatus / enzymology*
  • Interphase
  • Mitosis
  • Protein Kinases / analysis*
  • Protein Kinases / immunology
  • Spindle Apparatus / enzymology*

Substances

  • Antibodies
  • Cyclic AMP
  • Protein Kinases