Extractability of polyphenols from black currant, red currant and gooseberry and their antioxidant activity

Acta Biol Hung. 2018 Jun;69(2):156-169. doi: 10.1556/018.69.2018.2.5.


In this study, we analyzed extracts of Ribes (black currant, red currant and gooseberry) fruits obtained with methanol, methanol 50% and water. For each extract total polyphenol content, total flavonoid content and total anthocyanin content was assessed. The antioxidant activity of extracts was evaluated by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging capacity and by the photo-chemiluminescence (PCL) method. Identification and quantification of individual phenolic compounds was performed by means of high performance liquid chromatograph coupled with diode array detector (HPLC-DAD) analyses. From each fruit, best extraction of polyphenols was obtained with methanol 50%. In case of red currants and gooseberry there was no significant difference in flavonoids and anthocyanins extraction rate by the different extraction solvents. For black currants the methanol and methanol 50% extract presented the highest antioxidant activity. For red currants extracts with methanol 50% showed stronger antioxidant activity (IC50 = 5.71 mg/ml for DPPH, IC50 = 1.17 mg/ml for ABTS) than those with methanol or water. In case of gooseberry by the DPPH test the water extract proved to be the most active (IC50 = 5.9 mg/ml). In the PCL test black currants methanol 50% extract was over 6 times more powerful as the ones from red currants. In case of gooseberries, water extract presented the highest antioxidant activity (41.84 μmol AAE/g). In black currant cyanidin-3-glucoside was the major compound. Quercetin 3-O-glucoside was identified in each sample. From cinnamic acid derivatives neochlorogenic acid was present in black currants in the highest amount (356.33 μg/g).

Keywords: Ribes nigrum L.; Ribes rubrum L.; Ribes uva-crispa L.; antioxidant activity; polyphenols.

MeSH terms

  • Anthocyanins / analysis
  • Antioxidants / pharmacology
  • Benzothiazoles
  • Biphenyl Compounds
  • Chlorogenic Acid / analogs & derivatives
  • Chlorogenic Acid / analysis
  • Chromatography, High Pressure Liquid
  • Free Radical Scavengers / pharmacology*
  • Glucosides / analysis
  • Indicators and Reagents
  • Luminescent Measurements
  • Picrates
  • Plant Extracts / analysis
  • Plant Extracts / pharmacology*
  • Polyphenols / analysis
  • Quercetin / analogs & derivatives
  • Quercetin / analysis
  • Quinic Acid / analogs & derivatives
  • Quinic Acid / analysis
  • Ribes
  • Sulfonic Acids


  • Anthocyanins
  • Antioxidants
  • Benzothiazoles
  • Biphenyl Compounds
  • Free Radical Scavengers
  • Glucosides
  • Indicators and Reagents
  • Picrates
  • Plant Extracts
  • Polyphenols
  • Sulfonic Acids
  • cyanidin-3-O-beta-glucopyranoside
  • quercetin 3'-O-glucoside
  • Quinic Acid
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • Chlorogenic Acid
  • Quercetin
  • 1,1-diphenyl-2-picrylhydrazyl
  • 5'-O-caffeoylquinic acid