Anti diabetic property of aqueous extract of Stevia rebaudiana Bertoni leaves in Streptozotocin-induced diabetes in albino rats

BMC Complement Altern Med. 2018 Jun 11;18(1):179. doi: 10.1186/s12906-018-2245-2.


Background: Stevia (Stevia rebaudiana) natural, non-caloric sugar substitute is rich source of pharmacologically important glycoside stevioside that is linked to the pathology and complications of diabetes.

Methods: The current research was carried out to explore the anti-diabetic effect of aqueous extract of Stevia rebaudiana leaves in albino rats. For this purpose, diabetes was induced by administration of streptozotocin (40 mg/kg body weight, intraperitoneally). The diabetic rats were administered with aqueous stevia extract at different dose levels (200, 300, 400 and 500 ppm/kg b.w) for 8 weeks; the control rats were fed basal diet during this period.

Results: Stevia aqueous extract improved caloric management and weight control by decreasing the feed intake and body weight gain. Furthermore, intake of stevia extract resulted in significant (P < 0.05) decrease in the random blood glucose level (- 73.24%) and fasting blood glucose (- 66.09%) and glycosylated (HbA1c) hemoglobin (5.32%) while insulin (17.82 μIU/mL) and liver glycogen (45.02 mg/g) levels significantly improved in the diabetic rats, compared with the diabetic and non-diabetic control rats after 8 weeks study period.

Conclusions: It is concluded that aqueous extact of stevia has anti-diabetic effects in albino rats, and therefore could be promising nutraceutical therapy for the management of diabetes and its associated complications.

Keywords: Diabetes; Fasting blood glucose; HbA1c; Insulin; Liver glycogen; Random blood glucose; Stevia rebaudiana bertoni; Stevioside.

MeSH terms

  • Animals
  • Blood Glucose / drug effects*
  • Diabetes Mellitus, Experimental / metabolism*
  • Eating / drug effects
  • Hypoglycemic Agents / pharmacology*
  • Insulin / blood
  • Male
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry
  • Rats
  • Stevia / chemistry*
  • Weight Gain / drug effects


  • Blood Glucose
  • Hypoglycemic Agents
  • Insulin
  • Plant Extracts