Stable expression of cDNA encoding the human interleukin 2 receptor in eukaryotic cells

J Exp Med. 1985 Jul 1;162(1):363-8. doi: 10.1084/jem.162.1.363.

Abstract

Human interleukin 2 (IL-2) receptor cDNA derived from HUT 102B2 cells was stably expressed in murine L cells. These L cell transfectants (a) displayed surface receptors of the aberrant size of the IL-2 receptors on HUT 102B2 cells, (b) did not respond to exogenous IL-2 with augmented proliferation, and (c) expressed low affinity but not high affinity receptors for IL-2.

MeSH terms

  • Animals
  • DNA / genetics*
  • Genetic Vectors
  • Humans
  • Interleukin-2 / immunology*
  • L Cells / immunology
  • Mice
  • Receptors, Immunologic / biosynthesis
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / metabolism
  • Receptors, Interleukin-2
  • Simian virus 40 / genetics
  • Transfection

Substances

  • Interleukin-2
  • Receptors, Immunologic
  • Receptors, Interleukin-2
  • DNA