Histological Observation of Teratogenic Phenotypes Induced in Frog Embryo Assays

Methods Mol Biol. 2018:1797:309-323. doi: 10.1007/978-1-4939-7883-0_16.

Abstract

Amphibian embryos have long served as an ideal model for teratogenicity testing. While whole-mount embryo observations can be utilized, histological observation of teratogenic phenotypes provides a wealth of additional information that can lead to mechanistic insights. In this chapter, detailed protocols for two methods of sectioning embryos as well as a guide for histological analysis is provided.

Keywords: Cryosectioning; Embryo; FETAX; Histology; Paraffin sectioning; Sectioning; Teratogen; Toxicology; Xenopus.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Biological Assay
  • Embryo, Nonmammalian / drug effects
  • Embryo, Nonmammalian / pathology*
  • Embryonic Development*
  • Histocytochemistry / methods*
  • Paraffin Embedding
  • Teratogens / toxicity*
  • Toxicity Tests / methods*
  • Xenopus laevis / embryology*
  • Xenopus laevis / physiology

Substances

  • Teratogens