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. 2018;1797:577-594.
doi: 10.1007/978-1-4939-7883-0_34.

Whole Mount In Situ Hybridization and Morphometric Analysis in Rabbit Embryos

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Whole Mount In Situ Hybridization and Morphometric Analysis in Rabbit Embryos

Yoshinori Kawamura et al. Methods Mol Biol. .

Abstract

Rabbits are valuable experimental animals used to evaluate the teratogenicity of chemical entities. If teratogenicity is recognized, it is very important to investigate the pathogenesis to assess the potential risk in humans. For this investigation, whole-mount in situ hybridization (WISH) is highly useful for visualizing spatial and temporal changes in the expression patterns of the targeted genes and or proteins. In this chapter, the WISH method in rabbit embryos, using a digoxigenin-labeled RNA probe, and the morphometric analysis of the expression pattern is described.

Keywords: Embryo; Rabbit; Thalidomide; WISH; Whole-mount in situ hybridization.

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