Whole Mount In Situ Hybridization and Morphometric Analysis in Rabbit Embryos

Yoshinori Kawamura; Kiyoshi Matsumoto

doi:10.1007/978-1-4939-7883-0_34

Whole Mount In Situ Hybridization and Morphometric Analysis in Rabbit Embryos

Methods Mol Biol. 2018:1797:577-594. doi: 10.1007/978-1-4939-7883-0_34.

Authors

Yoshinori Kawamura¹, Kiyoshi Matsumoto²

Affiliations

¹ Drug Safety Research Laboratories, Pharmaceutical Research Division, Takeda Pharmaceutical Company, Fujisawa, Kanagawa, Japan.
² Drug Safety Research Laboratories, Pharmaceutical Research Division, Takeda Pharmaceutical Company, Fujisawa, Kanagawa, Japan. kiyoshi.matsumoto@takeda.com.

PMID: 29896718
DOI: 10.1007/978-1-4939-7883-0_34

Abstract

Rabbits are valuable experimental animals used to evaluate the teratogenicity of chemical entities. If teratogenicity is recognized, it is very important to investigate the pathogenesis to assess the potential risk in humans. For this investigation, whole-mount in situ hybridization (WISH) is highly useful for visualizing spatial and temporal changes in the expression patterns of the targeted genes and or proteins. In this chapter, the WISH method in rabbit embryos, using a digoxigenin-labeled RNA probe, and the morphometric analysis of the expression pattern is described.

Keywords: Embryo; Rabbit; Thalidomide; WISH; Whole-mount in situ hybridization.

MeSH terms

Animals
Embryo, Mammalian*
Embryonic Development*
Female
In Situ Hybridization* / methods
Phenotype
Pregnancy
Rabbits