Three-dimensional structure of renal Na,K-ATPase determined by electron microscopy of membrane crystals

FEBS Lett. 1985 Jul 22;187(1):182-6. doi: 10.1016/0014-5793(85)81238-2.

Abstract

The three-dimensional structure of Na,K-ATPase was determined by electron microscopy and image processing. Tilt series of negatively stained membrane crystals were recorded. The projections were analyzed by Fourier methods and the data combined to a 3-D model. The unit cell contains two rod-shaped stain-deficient regions interpreted as alpha beta-promoters of Na,K-ATPase. The rods are related by dyad axes oriented perpendicular to the membrane. Outside the lipid bilayer the rods contact different protein units on the two sides of the membrane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Crystallization
  • Kidney Medulla / enzymology*
  • Macromolecular Substances
  • Microscopy, Electron
  • Models, Chemical
  • Rabbits
  • Sodium-Potassium-Exchanging ATPase*
  • X-Ray Diffraction

Substances

  • Macromolecular Substances
  • Sodium-Potassium-Exchanging ATPase