Leishmanicidal and fungicidal activity of lipases obtained from endophytic fungi extracts

PLoS One. 2018 Jun 18;13(6):e0196796. doi: 10.1371/journal.pone.0196796. eCollection 2018.

Abstract

This work describes the production of lipases from endophytic fungi: Vermisporium-like, Emericella nidulans, Dichotomophtora portulacae and D. boerhaaviae and the biological activity against the dermatophyte fungi Malassezia sp and Microsporum canis and the parasite Leishmania amazonensis. All fungal enzymes extract showed lipolysis action in the media that contains long carbon chain lipids. The proteomic analysis of lipases exhibits several molecules mostly ranging in size from 220 to 20 kDa, with clear differences in protein profile's yield. All fungal enzymes were competent to eliminate promastigote forms of Leishmania amazonensis at 5 mg.mL-1. The antileishmanial activity of lipases from Vermisporium-like, E. nidulans, D. portulacae and D. boerhaaviae in amastigote forms, promoted the reduction in viability of 78.88, 39.65, 63.17 and 98.13%, with selectivity index of 19.56, 30.68, 18.09 and 20.99. In relation to antifungal activity, Dichothomophtora enzymes demonstrate best action with MFC of 14.65 μg.mL-1 against Malassezia sp and Microsporum canis, respectively. These results allow us to infer that lipases from entophytic fungi displays activity against dermatophyte fungi (Malassezia sp. and Microsporum canis) as well as Leishmania.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antifungal Agents* / chemistry
  • Antifungal Agents* / pharmacology
  • Antiprotozoal Agents* / chemistry
  • Antiprotozoal Agents* / pharmacology
  • Arthrodermataceae / growth & development*
  • Complex Mixtures* / chemistry
  • Complex Mixtures* / pharmacology
  • Emericella* / chemistry
  • Emericella* / enzymology
  • Fungal Proteins* / chemistry
  • Fungal Proteins* / pharmacology
  • Leishmania / growth & development*
  • Lipase* / chemistry
  • Lipase* / pharmacology
  • Mice
  • Mice, Inbred BALB C

Substances

  • Antifungal Agents
  • Antiprotozoal Agents
  • Complex Mixtures
  • Fungal Proteins
  • Lipase

Grant support

This work was supported by CNPq (National Counseil for scientific and technological development), Project number: 421445/2016-5.