Occurrence and genetic characterization of Giardia duodenalis and Cryptosporidium spp. from adult goats in Sichuan Province, China

PLoS One. 2018 Jun 18;13(6):e0199325. doi: 10.1371/journal.pone.0199325. eCollection 2018.

Abstract

Giardia duodenalis and Cryptosporidium spp. are common gastrointestinal protozoa in mammals. Many studies have been conducted on the distribution of G. duodenalis and Cryptosporidium spp. genotypes in sheep and cattle. However, in China, information about molecular characterization and genetic analysis of G. duodenalis and Cryptosporidium spp. in goats is limited. In this study, 342 fecal samples from adult goats were collected from 12 farms in Sichuan Province, China. The occurrence of G. duodenalis and Cryptosporidium spp. in adult goats was 14.9% (51/342) and 4.7% (16/342), respectively. All G. duodenalis were identified as assemblage E, with two novel genotypes (assemblages E17 and E18) being detected at the beta-giardin (bg) locus. Based on three loci-beta-giardin (bg), triose phosphate isomerase (tpi), and glutamate dehydrogenase (gdh)-multilocus sequence typing revealed three novel multilocus genotypes (MLGs) of assemblage E (MLG-E1, E2, E3 (sc)). Small Subunit (SSU) rRNA-based PCR identified two Cryptosporidium species, namely C. xiaoi (11/16) and C. suis (5/16). This study is not only the first to report C. suis infection in adult goats in China but is also the first to use the MLG approach to identify G. duodenalis in adult goats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cryptosporidiosis / genetics
  • Cryptosporidiosis / parasitology*
  • Cryptosporidiosis / transmission
  • Cryptosporidium / genetics*
  • Cryptosporidium / isolation & purification
  • Cryptosporidium / pathogenicity
  • Genotype
  • Giardia lamblia / genetics*
  • Giardia lamblia / isolation & purification
  • Giardia lamblia / pathogenicity
  • Glutamate Dehydrogenase / genetics
  • Goats / genetics
  • Goats / parasitology*
  • Humans
  • Sequence Analysis, DNA
  • Triose-Phosphate Isomerase / genetics

Substances

  • Glutamate Dehydrogenase
  • Triose-Phosphate Isomerase

Grants and funding

This work was supported by the National Key Research and Development Program of China (2016YFD0501009); the National Natural Science Foundation of China (31272620); and the Chengdu Giant Panda Breeding Research Foundation (CPF2015-4).