Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins

Biophys J. 2018 Jul 3;115(1):22-25. doi: 10.1016/j.bpj.2018.05.024. Epub 2018 Jun 15.


Macrodiscs, which are magnetically alignable lipid bilayer discs with diameters of >30 nm, were obtained by solubilizing protein-containing liposomes with styrene-maleic acid copolymers. Macrodiscs provide a detergent-free phospholipid bilayer environment for biophysical and functional studies of membrane proteins under physiological conditions. The narrow resonance linewidths observed from membrane proteins in styrene-maleic acid macrodiscs advance structure determination by oriented sample solid-state NMR spectroscopy.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Lipid Bilayers / chemistry*
  • Magnets / chemistry
  • Maleates / chemistry*
  • Membrane Proteins / chemistry*
  • Nuclear Magnetic Resonance, Biomolecular*
  • Styrene / chemistry*


  • Lipid Bilayers
  • Maleates
  • Membrane Proteins
  • Styrene
  • maleic acid