PreDicta chip-based high resolution diagnosis of rhinovirus-induced wheeze

Nat Commun. 2018 Jun 18;9(1):2382. doi: 10.1038/s41467-018-04591-0.


Rhinovirus (RV) infections are major triggers of acute exacerbations of severe respiratory diseases such as pre-school wheeze, asthma and chronic obstructive pulmonary disease (COPD). The occurrence of numerous RV types is a major challenge for the identification of the culprit virus types and for the improvement of virus type-specific treatment strategies. Here, we develop a chip containing 130 different micro-arrayed RV proteins and peptides and demonstrate in a cohort of 120 pre-school children, most of whom had been hospitalized due to acute wheeze, that it is possible to determine the culprit RV species with a minute blood sample by serology. Importantly, we identify RV-A and RV-C species as giving rise to most severe respiratory symptoms. Thus, we have generated a chip for the serological identification of RV-induced respiratory illness which should be useful for the rational development of preventive and therapeutic strategies targeting the most important RV types.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asthma / immunology
  • Asthma / virology*
  • Child, Preschool
  • Female
  • Humans
  • Infant
  • Male
  • Picornaviridae Infections / diagnosis
  • Picornaviridae Infections / virology
  • Protein Array Analysis / instrumentation*
  • Protein Array Analysis / methods
  • Respiratory Sounds
  • Rhinovirus / classification*
  • Rhinovirus / immunology
  • Rhinovirus / isolation & purification
  • Serologic Tests / instrumentation
  • Viral Proteins / immunology*


  • Viral Proteins
  • viral protein 1, rhinovirus