A recurrent point mutation in PRKCA is a hallmark of chordoid gliomas

Nat Commun. 2018 Jun 18;9(1):2371. doi: 10.1038/s41467-018-04622-w.


Chordoid glioma (ChG) is a characteristic, slow growing, and well-circumscribed diencephalic tumor, whose mutational landscape is unknown. Here we report the analysis of 16 ChG by whole-exome and RNA-sequencing. We found that 15 ChG harbor the same PRKCA D463H mutation. PRKCA encodes the Protein kinase C (PKC) isozyme alpha (PKCα) and is mutated in a wide range of human cancers. However the hot spot PRKCA D463H mutation was not described in other tumors. PRKCA D463H is strongly associated with the activation of protein translation initiation (EIF2) pathway. PKCαD463H mRNA levels are more abundant than wild-type PKCα transcripts, while PKCαD463H is less stable than the PCKαWT protein. Compared to PCKαWT, the PKCαD463H protein is depleted from the cell membrane. The PKCαD463H mutant enhances proliferation of astrocytes and tanycytes, the cells of origin of ChG. In conclusion, our study identifies the hallmark mutation for chordoid gliomas and provides mechanistic insights on ChG oncogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Cell Proliferation
  • Cells, Cultured
  • Cerebral Ventricle Neoplasms / genetics*
  • Cerebral Ventricle Neoplasms / metabolism
  • Female
  • Glioma / genetics*
  • Glioma / metabolism
  • Humans
  • Male
  • Middle Aged
  • Point Mutation
  • Protein Kinase C-alpha / genetics*
  • Protein Kinase C-alpha / metabolism


  • PRKCA protein, human
  • Protein Kinase C-alpha