MicroRNA-1297 contributes to the progression of human cervical carcinoma through PTEN

Artif Cells Nanomed Biotechnol. 2018;46(sup2):1120-1126. doi: 10.1080/21691401.2018.1479711. Epub 2018 Jun 19.


Background: The human cervical carcinoma oncogenic mechanisms still remain elusive. Thus, we proposed to understand the biological role of a newly discovered therapeutic miRNA.

Methods: MiR-1297 related to human cervical carcinoma was selected for this study. TaqMan qRT- PCR assay was used to profile miRNA, phosphatase and tensin homolog (PTEN) expression in randomly chosen tumour with non-tumour tissues, and the apoptosis factors expression. Cell proliferation was monitored by CCK-8 assay and colony formation assay. Apoptosis was determined by flow cytometry. Protein level was determined by western blotting. 3'UTR was performed to validate the direct binding sites of miR-1297 on PTEN. SPSS was used for statistical analyses.

Results: MiR-1297 is repressed and PTEN activated in human cervical cancer tissues. After miR-1297 overexpression, HeLa cells had an increase in cell proliferation and decrease in apoptosis. PTEN expression is negatively correlation with miR-1297. PTEN silencing display the similar pattern as miRNA-1297 overexpression to inhibit HeLa cell growth and apoptosis in vitro.

Conclusions: Our data indicate that miR-1297 contribute to the human cervical carcinoma through PTEN. miR-1297 could be a reasonable miRNA for future studies.

Keywords: MiR-1297; PTEN; apoptosis; cell proliferation.

MeSH terms

  • Apoptosis / genetics
  • Cell Proliferation / genetics
  • Disease Progression*
  • Down-Regulation / genetics
  • Female
  • HeLa Cells
  • Humans
  • MicroRNAs / genetics*
  • PTEN Phosphohydrolase / genetics*
  • Up-Regulation / genetics
  • Uterine Cervical Neoplasms / genetics
  • Uterine Cervical Neoplasms / pathology*


  • MIRN1297 microRNA, human
  • MicroRNAs
  • PTEN Phosphohydrolase
  • PTEN protein, human