Prospective Evaluation of the ESPGHAN Guidelines for Diagnosis of Celiac Disease in New Zealand Children

J Pediatr Gastroenterol Nutr. 2018 Dec;67(6):749-754. doi: 10.1097/MPG.0000000000002065.


Objective: The 2012 European Society of Pediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) guideline for diagnosis of celiac disease (CD) questioned the requirement for intestinal biopsy to confirm the diagnosis. The guideline recommends that in symptomatic patients with consistent human leukocyte antigen (HLA) subtypes, the diagnostic accuracy of strongly positive serology is sufficient to confirm the diagnosis. We prospectively assessed these guidelines in a "real-life" clinical setting.

Methods: One hundred and four children referred for evaluation of possible CD were prospectively recruited. Following informed consent, blood was drawn for serological testing and HLA analysis at upper gastrointestinal endoscopy. Histological findings according to Marsh criteria were correlated with blood results and the accuracy of the guideline analyzed.The study also examined the role of deamidated gliadin peptide (DGP) in the diagnosis of CD.

Results: For symptomatic patients with consistent HLA subtypes, strongly positive serology (as described in the ESPGHAN guidelines) accurately predicted biopsy-proven CD in >95% of cases. DGP was positive in fewer patients than anti-TG2 or EMA. Incorporation of DGP as a second confirmatory serological test in place of EMA was associated with maintained predictive value of guideline, but fewer patients fulfilling criteria for biopsy-free diagnosis.

Conclusions: The ESPGHAN guideline performs well in our population. Adoption of the guideline would reduce the number of patients requiring endoscopy without compromise in diagnostic accuracy. The involvement of pediatric gastroenterological expertise, however, remains key to diagnosis of CD.

Publication types

  • Evaluation Study

MeSH terms

  • Adolescent
  • Autoantibodies / blood
  • Autoantibodies / immunology
  • Biomarkers / blood
  • Celiac Disease / diagnosis*
  • Child
  • Child, Preschool
  • Female
  • GTP-Binding Proteins / immunology
  • Gastroenterology / standards*
  • Gliadin / blood
  • HLA Antigens / blood
  • Humans
  • Male
  • New Zealand
  • Nutritional Sciences / standards*
  • Pediatrics / standards*
  • Peptides / blood
  • Practice Guidelines as Topic
  • Predictive Value of Tests
  • Prospective Studies
  • Serologic Tests / standards
  • Serologic Tests / statistics & numerical data*
  • Transglutaminases / immunology


  • Autoantibodies
  • Biomarkers
  • HLA Antigens
  • Peptides
  • Gliadin
  • transglutaminase 2
  • Transglutaminases
  • GTP-Binding Proteins