The Structure and Evolution of a 461 Amino Acid Human Protein C Precursor and Its Messenger RNA, Based Upon the DNA Sequence of Cloned Human Liver cDNAs

Nucleic Acids Res. 1985 Jul 25;13(14):5233-47. doi: 10.1093/nar/13.14.5233.

Abstract

Human liver cDNA coding for protein C has been synthesized, cloned and sequenced. The abundance of protein C message is approximately 0.02% of total mRNA. Three overlapping clones contain 1,798 nucleotides of contiguous sequence, which approximates the size of the protein's mRNA, based upon Northern hybridization. The cDNA sequence consists of 73 5'-noncoding bases, coding sequence for a 461 amino acid nascent polypeptide precursor, a TAA termination codon, 296 3'-noncoding bases, and a 38 base polyadenylation segment. The nascent protein consists of a 33 amino acid "signal", a 9 amino acid propeptide, a 155 amino acid "light" chain, a Lys-Arg connecting dipeptide, and a 262 amino acid "heavy" chain. Human protein C and Factor IX and X precursors possess about one third identical amino acids (59% in the gamma-carboxyglutamate domain), including two forty-six amino acid segments homologous to epidermal growth factor. Human protein C also has similar homology with prothrombin in the "leader", gamma-carboxyglutamate and serine protease domains, but lacks the two "kringle" domains found in prothrombin.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA / analysis*
  • DNA Restriction Enzymes / metabolism
  • Deoxyribonuclease BamHI
  • Deoxyribonucleases, Type II Site-Specific*
  • Factor IX / analysis
  • Factor X / analysis
  • Glycoproteins / biosynthesis*
  • Humans
  • Nucleic Acid Hybridization
  • Protein C
  • Protein Precursors / genetics*
  • Prothrombin / analysis
  • RNA, Messenger / analysis*

Substances

  • Glycoproteins
  • Protein C
  • Protein Precursors
  • RNA, Messenger
  • Prothrombin
  • Factor IX
  • Factor X
  • DNA
  • DNA Restriction Enzymes
  • Deoxyribonuclease BamHI
  • CTGCAG-specific type II deoxyribonucleases
  • Deoxyribonucleases, Type II Site-Specific
  • GCGC-specific type II deoxyribonucleases
  • GTCGAC-specific type II deoxyribonucleases

Associated data

  • GENBANK/X02750