Fractionation of red diamond rattlesnake (Crotalus ruber ruber) venom: protease, phosphodiesterase, L-amino acid oxidase activities and effects of metal ions and inhibitors on protease activity

Toxicon. 1985;23(2):337-40. doi: 10.1016/0041-0101(85)90157-6.

Abstract

Crotalus ruber ruber venom contains several different proteases, and the proteolytic activity of the crude venom is 6-15 times greater in adult than in juvenile venom. Venom samples were assayed for proteolytic, phosphodiesterase, L-amino acid oxidase and elastinase-like activities and were subjected to gel filtration on BioGel P-100. Two major size classes of proteases were resolved (mol. wt 67,000 and 20,500). EDTA, N-ethylmaleimide (N-EM) and 1,10-phenanthroline inhibited proteolytic activity of crude venom, and EDTA, Zn2+ and Cu2+ inhibited proteolytic activity of the fractionated venom.

MeSH terms

  • Amino Acid Oxidoreductases / analysis*
  • Animals
  • Chromatography, Gel
  • Crotalid Venoms / analysis*
  • L-Amino Acid Oxidase
  • Metals / pharmacology*
  • Peptide Hydrolases / analysis*
  • Phosphoric Diester Hydrolases / analysis*
  • Protease Inhibitors / pharmacology*

Substances

  • Crotalid Venoms
  • Metals
  • Protease Inhibitors
  • Amino Acid Oxidoreductases
  • L-Amino Acid Oxidase
  • Phosphoric Diester Hydrolases
  • Peptide Hydrolases