Purification of DNA From Formaldehyde Fixed and Paraffin Embedded Human Tissue

Biochem Biophys Res Commun. 1985 Jul 16;130(1):118-26. doi: 10.1016/0006-291x(85)90390-0.

Abstract

The ability to isolate DNA from preserved human tissues would provide numerous experimental opportunities. In this report it is shown that DNA can be extracted from tissues prepared for routine histopathological examination (i.e., fixed with formaldehyde and embedded in paraffin). Although the extracted DNA is not intact, it is double stranded, cleavable with restriction endonucleases, and suitable for a variety of standard techniques used in molecular biology.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carcinoma / analysis
  • Colonic Neoplasms / analysis
  • DNA / analysis
  • DNA Restriction Enzymes
  • DNA, Neoplasm / isolation & purification*
  • Fixatives
  • Formaldehyde*
  • Humans
  • Nucleic Acid Hybridization
  • Placenta / analysis

Substances

  • DNA, Neoplasm
  • Fixatives
  • Formaldehyde
  • DNA
  • DNA Restriction Enzymes