A novel tumor-associated molecular species of 5'-nucleotide phosphodiesterase

Biochem Biophys Res Commun. 1985 Aug 15;130(3):1072-7. doi: 10.1016/0006-291x(85)91725-5.

Abstract

5'-Nucleotide phosphodiesterase (5'-NPDase) was partially purified from plasma membranes of murine lymphoma L5178Y. The enzyme was inactivated by N-ethylmaleimide and Zn2+, but stabilized by dithiothreitol, suggesting that it is an SH enzyme. The enzyme, Km 1.54 mM, pI 5.8 and MW 23k, differs from liver 5'-NPDase in MW, Km and sensitivity to some inhibitors. On the contrary, 5'-NPDase, derived from normal mouse organs, is similar to the liver enzyme. The results suggest that tumor cells possess a novel molecular species of 5'-NPDase.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Membrane / enzymology
  • Kinetics
  • Leukemia L5178 / enzymology*
  • Leukemia, Experimental / enzymology*
  • Liver / enzymology
  • Mice
  • Mice, Inbred DBA
  • Molecular Weight
  • Neoplasms, Experimental / enzymology
  • Phosphodiesterase I
  • Phosphoric Diester Hydrolases / isolation & purification
  • Phosphoric Diester Hydrolases / metabolism*
  • Tissue Distribution

Substances

  • Phosphoric Diester Hydrolases
  • Phosphodiesterase I