Evaluation of Two New Membrane-Based and Microtiter Plate Enzyme-Linked Immunosorbent Assays for Detection of Campylobacter jejuni in Stools of Bangladeshi Children

J Clin Microbiol. 2018 Aug 27;56(9):e00702-18. doi: 10.1128/JCM.00702-18. Print 2018 Sep.


Two new monoclonal antibody-based, sandwich enzyme immunoassays (EIAs) for fecal antigen detection of Campylobacter jejuni or Campylobacter coli were evaluated using diarrheal stool specimens from a cohort of children in Bangladesh. These children routinely harbor multiple enteric pathogens, often at levels that make it difficult to assign diarrheal symptoms to a causative agent. A panel of 158 PCR-positive specimens with a broad range of C. jejuni/C. coli DNA cycle threshold (CT ) values was used to assess the ability of the two tests to detect C. jejuni/C. coli antigen amounts that varied widely. A panel of 100 C. jejuni/C. coli PCR-negative specimens was used to verify that the assays correctly identified specimens as negative when the sample contained other enteric pathogens. Further analysis was conducted on a subset of 46 specimens that contained particularly substantial amounts of C. jejuni/C. coli (CT of ≤19.7) that previous studies have ascribed as "diarrhea-associated." The Quik Chek rapid EIA and the Chek enzyme-linked immunosorbent assays (ELISAs) had a sensitivity of 95.7% for these specimens (specificities, 97% and 96%, respectively), supporting the usefulness of the new Chek and Quik Chek assays in symptomatic presentations, where Campylobacter is the likely etiology.

Keywords: Campylobacter; ELISA; Quik Chek; diagnostic.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial / analysis
  • Bacteriological Techniques / methods*
  • Bangladesh
  • Campylobacter Infections / diagnosis*
  • Campylobacter coli / immunology
  • Campylobacter coli / isolation & purification*
  • Campylobacter jejuni / immunology
  • Campylobacter jejuni / isolation & purification*
  • Child, Preschool
  • Diagnostic Tests, Routine
  • Diarrhea / diagnosis
  • Enzyme-Linked Immunosorbent Assay*
  • Feces / microbiology*
  • Humans
  • Infant
  • Infant, Newborn
  • Polymerase Chain Reaction / standards
  • Sensitivity and Specificity


  • Antigens, Bacterial