Identification of Phosphorylated Proteins on a Global Scale

Curr Protoc Chem Biol. 2018 Sep;10(3):e48. doi: 10.1002/cpch.48. Epub 2018 May 31.


Liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) has enabled researchers to analyze complex biological samples with unprecedented depth. It facilitates the identification and quantification of modifications within thousands of proteins in a single large-scale proteomic experiment. Analysis of phosphorylation, one of the most common and important post-translational modifications, has particularly benefited from such progress in the field. Here, detailed protocols are provided for a few well-regarded, common sample preparation methods for an effective phosphoproteomic experiment. © 2018 by John Wiley & Sons, Inc.

Keywords: LC-MS; phosphopeptide enrichment; phosphoproteomics; protein phosphorylation; proteomics; sample preparation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Chromatography, Liquid
  • Phosphorylation
  • Protein Processing, Post-Translational
  • Proteins / analysis*
  • Proteins / chemistry*
  • Proteins / metabolism
  • Tandem Mass Spectrometry


  • Proteins