Generation and Identification of Mutations Resulting in Chronic and Age-Related Phenotypes in Mice

Curr Protoc Mouse Biol. 2018 Jun;8(2):e42. doi: 10.1002/cpmo.42. Epub 2018 May 21.

Abstract

Aging is inevitable, and our society must deal with the consequences: namely, an increased incidence of disease and ill health. Many mouse models of disease are acute or early onset or are induced in young mice, despite the fact that aging is a significant risk factor for a range of significant diseases. To improve modeling of such diseases, we should incorporate aging into our models. Many systems are affected by aging, with a decline in mitochondrial function, an increase in senescence, a loss of resilience, telomere shortening, and a decline in immune function being key factors in the increased susceptibility to disease that is associated with aging. To develop novel models of age-related disease, we undertook a phenotype-driven screen of a pipeline of mutagenized mice. Here, we describe some of the underlying protocols and outline important aspects to consider when studying aged mice. © 2018 by John Wiley & Sons, Inc.

Keywords: age-related disease; ageing; mutagenesis; phenotyping.

MeSH terms

  • Aging*
  • Animals
  • Disease Models, Animal*
  • Mice
  • Mutation*
  • Phenotype*