[The protective effects and mechanisms of Trillium tschonoskii Maxim on rats' liver damage induced by lipopolysaccharide]

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2016 Apr 8;32(4):373-377. doi: 10.13459/j.cnki.cjap.2016.04.022.
[Article in Chinese]

Abstract

Objective: To study the protective effects of Trillium tschonoskii maxim (TTM) on rats' oxidative stress and hepatotoxicity in-duced by lipopolysaccharide (LPS).

Methods: Sixty SD rats were randomly divided into TTM high, medium and low dose groups, model group, Dexamethasone (DEX) control group and blank control group with ten rats in each group. The TTM high, medium and low dose groups were treated with 8, 4, 2 g/(kg·d) TTM by intragastric administration and model group, DEX control group and blank control group were treated with the same amount of distilled water respectively. The TTM high, medium and low dose groups, model group, DEX control group were injected intraperitoneal with 1 mg/kg LPS and the DEX control group was injected intraperitoneal with 5 mg/kg DEX, the blank control group was injected with same amount of normal saline every five days. The indexes of rats' thymus and spleen were measured in 30 days. The activities of serum nitric oxide synthase (NOS), superoxide dismutase (SOD) and the contents of nitric oxide (NO), glutathione (GSH), glu-cosinolates barbituric acid reaction product(TBARS), white cells interleukin-6(IL-6), IL-10 and tumor necrosis factor-α(TNF-α), the activi-ties of liver SOD, GSH-Px and the contents of GSH and TBARS were measured.

Results: TTM high dose group was significantly different in body weight in 19~30 days(P<0.05); The index of thymus in TTM high, medium and low dose groups and the index of spleen in TTM high dose group were decreased significantly compared with those of the model group. The activity of serum NOS and the contents of TBARS and NO in TTM high, medium and low dose groups were decreased significantly(P<0.05). The activity of serum SOD in TTM high dose group and the contents of GSH in TTM medium and low dose groups were increased significantly(P<0.05). The contents of serum IL-6 and TNF-αin TTM high, medium dose groups were decreased significantly and the contents of serum IL-10 were increased significantly(P<0.05). The con-tents of liver TBARS in TTM high, medium dose groups were decreased significantly. The activity of liver SOD in TTM high, medium and low dose groups, the activity of GSH-Px in TTM high, medium dose groups and the contents of GSH in TTM high dose group were increased signifi-cantly(P<0.05).

Conclusions: TTM has a certain effect to delay the rats' atrophy of thymus and spleen generated by LPS. It can effectively reduce the activity of NOS in serum, reduce the formation of NO, improve the activity of SOD, GSH-Px and the contents of GSH, reduce lipid peroxidation, decrease the excessive secretion of IL-6, TNF-α, increase the contents of IL-10, which can resist inflammation and protect the liver.

Keywords: Trillium tschonoskii Maxim(traditional Chinese medicine); anti-in-flammatory; antioxidant; lipopolysaccharide; liver damage.

MeSH terms

  • Animals
  • Chemical and Drug Induced Liver Injury / drug therapy*
  • Cytokines / blood
  • Glutathione Peroxidase / metabolism
  • Lipopolysaccharides
  • Liver / drug effects
  • Nitric Oxide / blood
  • Nitric Oxide Synthase / blood
  • Plant Extracts / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Superoxide Dismutase / metabolism
  • Thiobarbituric Acid Reactive Substances / analysis
  • Trillium / chemistry*

Substances

  • Cytokines
  • Lipopolysaccharides
  • Plant Extracts
  • Thiobarbituric Acid Reactive Substances
  • Nitric Oxide
  • Glutathione Peroxidase
  • Nitric Oxide Synthase
  • Superoxide Dismutase