The use of a random priming procedure to generate cDNA libraries of infectious bronchitis virus, a large RNA virus

J Virol Methods. 1985 Jul;11(3):265-9. doi: 10.1016/0166-0934(85)90116-8.


An efficient method for the generation of gene banks from large RNA viruses is described using infectious bronchitis virus as an example. Randomly primed clones have been characterized and found to be representative of the viral genome including 5' leader sequences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular*
  • Coronaviridae / genetics*
  • DNA / biosynthesis*
  • Genes, Viral*
  • Infectious bronchitis virus / genetics*
  • Nucleic Acid Hybridization
  • Oligodeoxyribonucleotides / biosynthesis
  • Plasmids


  • Oligodeoxyribonucleotides
  • DNA