Driven by the environmental benefits that bio-bleaching could bring, the interest in xylanase has received enormous attention and hence, the search of xylanase with properties like no cellulase activity, function at elevated temperatures and pH continues. The present study reports the production of extracellular xylanase from Aspergillus nidulans using waste agro-residues as substrate. The optimum temperature (60 °C) and pH (9.0), classified the xylanase as thermo and alkali tolerant. The addition of salt of Mn2+ increased the xylanase activity to almost double; however, these ions were unable to protect the enzyme from thermal inactivation. The FTIR spectra of bamboo pulp treated with this xylanase, revealed reduction in lignin as evident from reduced peak intensity coupled with the reduction in kappa number. The SEM image of enzyme treated pulp, exhibited dissociation in fibers exposing the internal structure with slight roughness. Swelling was also observed there by increasing its thickness which eventually helped in improving its physical properties. The bleaching efficacy of indigenous xylanase as indicated in this study, has established its competence as a promising candidate for pre-treating the bamboo pulp.
Keywords: Aspergillus nidulans; Bamboo pulp bleaching; FT-IR; Manganese sulfate; Xylanase.
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