Nucleotide sequence of a spectinomycin adenyltransferase AAD(9) determinant from Staphylococcus aureus and its relationship to AAD(3") (9)

Mol Gen Genet. 1985;200(1):33-9. doi: 10.1007/BF00383309.


The nucleotide sequence of the spc determinant of the Staphylococcus aureus transposon Tn554 has been determined. This gene encodes a spectinomycin adenyltransferase, AAD(9), that mediates resistance to spectinomycin but not to streptomycin. The sequence predicts a 260 amino acid protein of molecular weight 28,943. A spectinomycin-sensitive mutant (spc-1) contains a G----A transition resulting in substitution of threonine (ACA) for alanine (GCA) at residue 165. The predicted amino acid sequence is 36% homologous to that of a widely distributed, gram-negative streptomycin/spectinomycin adenyltransferase, AAD(3") (9), specified by the aadA determinant (Holingshead and Vapnek 1985).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA Restriction Enzymes
  • Genes*
  • Genes, Bacterial*
  • Microbial Sensitivity Tests
  • Molecular Weight
  • Mutation
  • Nucleic Acid Conformation
  • Nucleotidyltransferases / genetics*
  • Plasmids
  • Protoplasts / enzymology
  • Spectinomycin / pharmacology
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / enzymology*
  • Staphylococcus aureus / genetics
  • Transcription, Genetic


  • Spectinomycin
  • Nucleotidyltransferases
  • streptomycin-spectinomycin adenylyltransferase
  • DNA Restriction Enzymes

Associated data

  • GENBANK/K02987