Nucleotide sequence of the Bacillus subtilis xylose isomerase gene: extensive homology between the Bacillus and Escherichia coli enzyme

Nucleic Acids Res. 1985 Aug 12;13(15):5717-22. doi: 10.1093/nar/13.15.5717.

Abstract

The xylose isomerase gene from Bacillus subtilis was cloned from a genomic BamH1 library by complementation of an isomerase defective Escherichia coli strain as previously described. The ATG initiation codon is preceded by a Shine-Dalgarno sequence and two hexamers being characteristic for the promoter region of Bacillus genes. The structural gene consists of 1320 base pairs, thus coding for a polypeptide chain of 440 amino acids with a molecular weight of 49 680. The polypeptide primary structure shows over 50% homology to that of the E. coli xylose isomerase.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldose-Ketose Isomerases*
  • Bacillus subtilis / enzymology*
  • Bacillus subtilis / genetics
  • Base Sequence
  • Carbohydrate Epimerases / genetics*
  • Codon
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Operon
  • Phosphotransferases (Alcohol Group Acceptor)*
  • Phosphotransferases / genetics

Substances

  • Codon
  • Phosphotransferases
  • Phosphotransferases (Alcohol Group Acceptor)
  • xylulokinase
  • Carbohydrate Epimerases
  • Aldose-Ketose Isomerases
  • xylose isomerase

Associated data

  • GENBANK/X01109
  • GENBANK/X02795