Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor

Nucleic Acids Res. 1985 Aug 26;13(16):5869-82. doi: 10.1093/nar/13.16.5869.

Abstract

DNA sequence complementary to the mRNA for rabbit interleukin-1 precursor (preIL-1) has been cloned from the cDNA library constructed using partially purified poly(A)+RNA from induced rabbit alveolar macrophages by mRNA hybridization-translation assay. By using this cDNA as a probe, human IL-1 cDNA was isolated from the cDNA library prepared using poly(A)+RNA from induced HL-60 cells, a human monocyte-like cell line. The amino acid sequences of the human and rabbit preIL-1 deduced from the cDNA sequences reveal their primary structures which consists of 271 and 267 amino acid residues, respectively. The amino acid sequence is 64% conserved between human and rabbit. The difference in number of amino acid residues results from the carboxy-terminal extention of 4 amino acid residues in human preIL-1. Expression of the cloned human cDNA in E. coli yielded biologically active IL-1.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular*
  • DNA / isolation & purification*
  • DNA Restriction Enzymes
  • Escherichia coli / genetics
  • Humans
  • Interleukin-1 / genetics*
  • Macrophages / metabolism
  • Nucleic Acid Hybridization
  • Plasmids
  • Protein Precursors / genetics*
  • RNA, Messenger / genetics
  • Rabbits
  • Species Specificity

Substances

  • Interleukin-1
  • Protein Precursors
  • RNA, Messenger
  • interleukin 1 precursor
  • DNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X02851
  • GENBANK/X02852