Aclidinium bromide inhibits human glioma cell proliferation, migration and invasion and promotes apoptosis via the PI3K/AKT signaling pathway

Neoplasma. 2018 Nov 15;65(6):865-871. doi: 10.4149/neo_2018_171117N705. Epub 2018 Jun 17.


This study investigates the anti-cancer potential of Aclidinium bromide (INN) in glioblastoma. Glioblastoma cell lines U251 and U87 were treated with INN and its effects on cell migration and invasion were assessed by transwell migration and invasion assays., The effects of INN on proliferation and apoptosis were detected by CCK-8 kit and flow cytometry, and Western blotting determined anti-apoptotic proteins and signaling pathway changes. The results show that INN effectively suppressed proliferation, migration and invasion and induced apoptosis in U251 and U87 cells, respectively. Furthermore, the expression levels of the Bcl-2 anti-apoptotic protein was significantly decreased while Bax and caspase-3 expression were both increased in glioblastoma cells (all, p<0.05). Moreover, INN inactivated the PI3K/AKT signaling pathway by down-regulating the level of p-AKT, p-mTOR, P70 and CyclinD1 (all, p<0.05). In conclusion, our data suggests that INN could provide novel anticancer therapy in the treatment of glioblastoma.

MeSH terms

  • Apoptosis
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Glioma / pathology*
  • Humans
  • Neoplasm Invasiveness
  • Phosphatidylinositol 3-Kinases*
  • Proto-Oncogene Proteins c-akt*
  • Signal Transduction / drug effects*
  • Tropanes / pharmacology*


  • Tropanes
  • Phosphatidylinositol 3-Kinases
  • Proto-Oncogene Proteins c-akt
  • aclidinium bromide