Inhibition of human leukocyte metabolism and random mobility by local anaesthesia

Acta Anaesthesiol Scand. 1985 Jul;29(5):520-3. doi: 10.1111/j.1399-6576.1985.tb02246.x.

Abstract

Hexose-monophosphate shunt (HMS) activity, myelo-peroxidase-(MPO)-mediated iodination and random mobility in human polymorphonuclear leukocytes (PMNs) were studied in the presence of lignocaine. Incubating the PMNs with 0.1% lignocaine during phagocytosis inhibited the 14CO2 produced from glucose-1-14-C via the HMS shunt by 33%. On increasing the concentration of lignocaine, a dose-dependent inhibition was noted. The MPO-mediated iodination was inhibited by 73% in the presence of 0.1% lignocaine, and complete inhibition took place when the concentration was increased to 0.5%. The random mobility of leukocytes was studied by an opto-electronic technique. In the presence of 0.5% lignocaine, all leukocytes examined were completely immobilized; in the presence of 0.1% lignocaine immobilization took place within 45-65 min.

MeSH terms

  • Anesthetics, Local / pharmacology*
  • Cell Migration Inhibition
  • Chemotaxis, Leukocyte / drug effects*
  • Depression, Chemical
  • Dose-Response Relationship, Drug
  • Humans
  • Lidocaine / pharmacology*
  • Neutrophils / drug effects*
  • Neutrophils / immunology
  • Neutrophils / metabolism
  • Pentose Phosphate Pathway / drug effects
  • Peroxidase / antagonists & inhibitors
  • Phagocytosis / drug effects

Substances

  • Anesthetics, Local
  • Lidocaine
  • Peroxidase