Aberrant Expression of miR-142-3p and its Target Gene HMGA1 and FZD7 in Breast Cancer and its Clinical Significance

Clin Lab. 2018 Jun 1;64(6):915-921. doi: 10.7754/Clin.Lab.2017.171114.

Abstract

Background: Breast cancer is the second leading cause of cancer-related death among women worldwide. The aim of this study is to investigate the role of miR-142-3p in breast cancer cells and the related mechanism.

Methods: Sixty paired breast cancer tissues were collected and 60 breast tissues from patients with mammary hyperplasia served as the control group. The expression of miR-142-3p was examined using RT-qPCR methods; moreover, we also performed receiver operating characteristic (ROC) curve analysis to determine whether miR142-3p can distinguish breast cancer patients from the controls. Next, HMGA1 and FZD7 have been predicted as target genes of miR-142-3p, and the expressions of HMGA1 and FZD7 in breast cancer tissue and the control group were examined using RT-qPCR and western blot methods.

Results: miR-142-3p was significantly down-regulated in breast cancer tissue compared with the controls, and the levels of miR-142-3p was negatively correlated with the tumor size, degree of differentiation, and metastasis (p < 0.01). Moreover, results of ROC curve analysis indicated that the expression of miR-142-3p can distinguish between patients with breast cancer and the control group (AUC = 0.819, 95% CI, 0.756 - 0.881). Furthermore, the expressions of HMGA1 and FZD7 were significantly up-regulated in patients with breast cancer compared with the controls. The level of miR-142-3p was negatively correlated with expressions of HMGA1 (r = -0.3507, p = 0.006) and FZD7 (r = -0.3410, p = 0.0077) in patients with breast cancer.

Conclusions: Our results proved that miR-142-3p may serve as a tumor suppressor in breast cancer by suppressing the expression of oncogene HMGA1 and FZD7, suggesting that miR-142-3p has the potential to become a diagnostic marker and therapeutic target for the early diagnosis and treatment of breast cancer.

MeSH terms

  • Adolescent
  • Adult
  • Biomarkers, Tumor / genetics
  • Breast / metabolism
  • Breast / pathology
  • Breast Neoplasms / diagnosis
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism
  • Diagnosis, Differential
  • Female
  • Frizzled Receptors / genetics*
  • Frizzled Receptors / metabolism
  • Gene Expression Regulation, Neoplastic*
  • HMGA1a Protein / genetics*
  • HMGA1a Protein / metabolism
  • Humans
  • Hyperplasia / diagnosis
  • Hyperplasia / genetics
  • Hyperplasia / metabolism
  • MicroRNAs / genetics
  • Young Adult

Substances

  • Biomarkers, Tumor
  • FZD7 protein, human
  • Frizzled Receptors
  • MIRN142 microRNA, human
  • MicroRNAs
  • HMGA1a Protein