Background: LC-3 and P62, two of important autophagy-related proteins, were reported highly expressed in many kinds of human malignancies and associated with outcomes of the patients. The purpose of this study was to investigate the expression status of LC-3 and P62 in non-small cell lung cancer patients and define the clinical-pathologic features.
Methods: 66 cases of non-small cell lung cancer patients were employed. The expression of LC-3 and P62 were detected by immunohistochemistry.
Results: LC-3 was positive stained in 27 out of 66 cases (40.9%) and P62 was positive stained in 43 out of 66 cases (65.2%). LC-3 positive staining was more frequently in squamous cell carcinoma patients (P<0.05); while P62 positive staining was more frequently in late-stage adenocarcinoma patients with metastasis (P<0.05). There was a significant negative correlation between LC-3 and P62 expressions in non-small cell lung cancer tissues (rs=-0.065, P<0.001). Kaplan-Meier analysis showed that patients with positive LC-3 expression had favorable clinical outcomes compared with the patients with negative LC-3 expression (P<0.05).
Conclusions: LC-3 and P62 showed abnormal expression in non-small cell lung cancer tissues, suggesting that autophagy is involved in the occurrence and development of NSCLC.
【中文题目：LC-3和P62在非小细胞肺癌中的表达 及其临床特征分析】 【中文摘要：背景与目的 LC-3和P62是参与自噬过程的两个重要蛋白，在许多恶性肿瘤中高表达并与预后有关。本研究的目的在于探讨自噬标记物LC-3、P62蛋白在非小细胞肺癌中的表达及其临床特征。方法 应用免疫组化法检测66例非小细胞肺癌患者肿瘤组织中LC-3和P62的表达情况，分析其表达状态与临床特征的关系。结果 LC-3在非小细胞肺癌组织中阳性表达率为40.9%（27/66），P62在非小细胞肺癌组织中阳性表达率为65.2%（43/66）。LC-3的阳性表达与患者的病理类型及转移情况明显相关（P<0.05）；而P62的阳性表达与患者的病理类型，临床分期及淋巴结转移明显相关（P<0.05）。LC-3和P62的阳性表达与患者的性别、年龄和吸烟史无明显相关性（P>0.05）。LC-3与P62在非小细胞肺癌组织中的表达呈负相关性（rs=-0.065, P<0.001）。Kaplan-Meier分析显示LC-3蛋白阳性表达的患者5年生存率明显高于阴性表达的患者（P<0.05），而P62蛋白的阳性表达确与患者的生存率无显著相关性。结论 LC-3和P62在非小细胞肺癌组织中呈现异常表达，提示自噬参与了非小细胞肺癌的发生及发展过程。】 【中文关键词：LC-3；P62；自噬；非小细胞肺癌；免疫组织化学】.
Keywords: Autophagy; Immunohistochemistry; LC-3; Non-small cell lung cancer; P62.