Vasopressin induced production of inositol trisphosphate and calcium efflux in a smooth muscle cell line

Biochem Biophys Res Commun. 1985 Aug 30;131(1):469-76. doi: 10.1016/0006-291x(85)91826-1.


Phosphatidylinositol metabolism and 45Ca2+ efflux were examined in a vascular smooth muscle cell line (A7r5). [Arg 8]Vasopressin stimulated the rapid formation (measurable at 1 sec) of inositol phosphates in a concentration-dependent manner. The time course for formation of inositol phosphates was similar to that for 45Ca2+ efflux from preloaded cells. The efflux of 45Ca2+ in response to [Arg8]vasopressin could be inhibited by a vasopressin antagonist. This supports the hypothesis that inositol 1,4,5-trisphosphate plays a role in vasopressin stimulated calcium mobilisation from an intracellular source in cultured vascular smooth muscle cells.

MeSH terms

  • Animals
  • Arginine Vasopressin / analogs & derivatives
  • Arginine Vasopressin / antagonists & inhibitors
  • Arginine Vasopressin / pharmacology*
  • Calcium / metabolism*
  • Calcium Radioisotopes / metabolism
  • Cell Line
  • Chlorides / pharmacology
  • Inositol 1,4,5-Trisphosphate
  • Inositol Phosphates / biosynthesis*
  • Kinetics
  • Lithium / pharmacology
  • Lithium Chloride
  • Muscle, Smooth, Vascular / drug effects
  • Muscle, Smooth, Vascular / metabolism*
  • Rats
  • Sugar Phosphates / biosynthesis*


  • Calcium Radioisotopes
  • Chlorides
  • Inositol Phosphates
  • Sugar Phosphates
  • Arginine Vasopressin
  • argipressin, beta-mercapto beta,beta-cyclopentamethylenepropionic acid(1)-O-methyl-Tyr(2)-Val(4)-
  • Inositol 1,4,5-Trisphosphate
  • Lithium
  • Lithium Chloride
  • Calcium