Puerarin, an isoflavone compound extracted from Gegen (Radix Puerariae Lobatae), modulates sclera remodeling caused by extremely low frequency electromagnetic fields

J Tradit Chin Med. 2016 Oct;36(5):678-82. doi: 10.1016/s0254-6272(16)30089-9.

Abstract

Objective: To evaluate the protective effect of puerarin [an isoflavone compound extracted from Gegen (Radix Puerariae Lobatae)] in scleral remodeling induced by extremely low frequency electromagnetic fields (ELF-EMFs).

Methods: Human fetal scleral fibroblasts (HFSFs) were divided into 5 groups: (a) untreated controls; (b) cells treated with ELF-EMFs; (c) cells treated with ELF-EMFs and puerarin 0.1 μM; (d) cells treated with ELF-EMFs and puerarin 1 μM; (e) cells treated with ELF-EMFs and puerarin 10 μM. Cell proliferation activity was measured by the cell-counting kit-8 assay. Matrix metalloproteinase-2 (MMP-2) activity was measured by gelatin enzymography. MMP-2 and collagenⅠ(COL1A1) mRNA, protein expression were measured by Real-Time polymerase chain reaction , Western blot analysis, respectively.

Results: Puerarin reduced the inhibition in cell proliferation, MMP-2 activity, mRNA, protein expression of HFSFs exposed to ELF-EMFs and enhanced the COL1A1 mRNA and protein expression.

Conclusion: Puerarin was found to participate in the matrix remodeling process. It might be a potential agent for the treatment of extracellular matrix degradation of sclera associated with ocular conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Proliferation / drug effects
  • Cell Proliferation / radiation effects
  • Collagen Type I / genetics
  • Collagen Type I / metabolism
  • Drugs, Chinese Herbal / pharmacology*
  • Electromagnetic Fields
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Fibroblasts / radiation effects
  • Humans
  • Isoflavones / pharmacology*
  • Matrix Metalloproteinase 2 / genetics
  • Matrix Metalloproteinase 2 / metabolism
  • Pueraria / chemistry*
  • Sclera / cytology
  • Sclera / drug effects*
  • Sclera / embryology
  • Sclera / radiation effects*

Substances

  • Collagen Type I
  • Drugs, Chinese Herbal
  • Isoflavones
  • collagen type I, alpha 1 chain
  • Matrix Metalloproteinase 2
  • puerarin