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. 2018 Jun 27;13(6):e0199315.
doi: 10.1371/journal.pone.0199315. eCollection 2018.

Prevalence of Brucellosis Among Patients Attending Wau Hospital, South Sudan

Free PMC article

Prevalence of Brucellosis Among Patients Attending Wau Hospital, South Sudan

Nuol Aywel Madut et al. PLoS One. .
Free PMC article


Brucellosis is a zoonotic disease of public health importance; its prevalence varies globally. In low-income countries, brucellosis is an endemic and neglected disease affecting both animals and humans. This study was intended to establish brucellosis sero-prevalence among patients attending Wau hospital, South Sudan. Across sectional study, was done among randomly selected patients attending Wau hospital. Data was collected using questionnaires and laboratory investigations. Rose Bengal plate Test (RBPT), Serum agglutination test (SAT) and Competitive Enzyme Linked Immuno Sorbent Assay (c-ELISA) was used in the analysis of blood samples serially starting with RBPT which is more sensitive and least specific then SAT. c-ELISA test which is most specific and less sensitive compared to RBPT and SAT was then used to confirm presence of Brucella antibodies in the samples. A total of 416 participants out of 1664 were enrolled to this study. The majority of participants were between 7-to-76 years of age with mean age of 30.72 (SD+/- 12.83). The sero-positivity of patient's blood samples for brucellosis using c-ELISA was 23.3% (97/416) among patients presenting to Wau hospital. Socio-demographic characteristics, occupation, clinical signs of disease and types of animals reared by animal owners showed no significant correlation with occurrence of sero-positivity among patient's blood samples for brucellosis. While ethnicity (Nilotic), knowledge of zoonotic disease, and consumption of animal urine were statistically significant (p<0.05). The study found a high prevalence of brucellosis among febrile patients attending Wau hospital general outpatient clinic. There is need for co-ordination and collaboration between veterinary and health sectors of government to help prevent and control brucellosis in the region.

Conflict of interest statement

The authors have declared that no competing interests exist.


Fig 1
Fig 1. Summary of the outcomes of patient recruitment in the study.

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    1. Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos EV. The new global map of human brucellosis. The Lancet infectious diseases. 2006;6(2):91–9. doi: 10.1016/S1473-3099(06)70382-6 - DOI - PubMed
    1. Muma J, Samui K, Oloya J, Munyeme M, Skjerve E. Risk factors for brucellosis in indigenous cattle reared in livestock–wildlife interface areas of Zambia. Preventive Veterinary Medicine. 2007;80(4):306–17. doi: 10.1016/j.prevetmed.2007.03.003 - DOI - PubMed
    1. McDermott JJ, Arimi S. Brucellosis in sub-Saharan Africa: epidemiology, control and impact. Veterinary microbiology. 2002;90(1):111–34. - PubMed
    1. Gruner E, Bernasconi E, Galeazzi R, Buhl D, Heinzle R, Nadal D. Brucellosis: an occupational hazard for medical laboratory personnel. Report of five cases. Infection. 1994;22(1):33–6. - PubMed
    1. Kunda J, Fitzpatrick J, Kazwala R, French NP, Shirima G, MacMillan A, et al. Health-seeking behaviour of human brucellosis cases in rural Tanzania. BMC Public Health. 2007;7(1):315. - PMC - PubMed

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Grant support

This work was supported by Makerere University-NORHED-CAPAZOMANINTECO Project UGA-13/0031 to NAM. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.