Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome

Chromosoma. 1985;92(4):290-6. doi: 10.1007/BF00329812.


We developed an aqueous spreading procedure that permits simultaneous analysis of human chromosomes by Q-banding and indirect immunofluorescence. Using this methodology and anticentromere antibodies from an autoimmune patient we compared the active and inactive centromeres of an isodicentric X chromosome. We show that a family of structurally related human centromere proteins (CENP-A, CENP-B, and CENP-C) is detectable only at the active centromere. These antigens therefore may be regarded both as morphological and functional markers for active centromeres.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Transformation, Viral
  • Centromere / analysis
  • Centromere / ultrastructure*
  • Chromosome Banding
  • Chromosomes / ultrastructure*
  • Female
  • Herpesvirus 4, Human / genetics
  • Humans
  • Karyotyping
  • Lymphocytes / cytology*
  • Metaphase
  • X Chromosome / ultrastructure*